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psumo1  (Addgene inc)


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    Structured Review

    Addgene inc psumo1
    Psumo1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/psumo1/pSUMO1+(Plasmid+%2352258)/bio_rxiv__2025__09__29__679180-149-18-31
    Average 92 stars, based on 10 article reviews
    psumo1 - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Construct:

    Article Title: SUMOylation of periplakin is critical for efficient reorganization of keratin filament network
    Article Snippet: .. Different PPL-C constructs were cloned in pGEX6P1 vector and cotransformed with pSUMO1 or pSUMO2 (Addgene). ..

    Clone Assay:

    Article Title: SUMOylation of periplakin is critical for efficient reorganization of keratin filament network
    Article Snippet: .. Different PPL-C constructs were cloned in pGEX6P1 vector and cotransformed with pSUMO1 or pSUMO2 (Addgene). ..

    Article Title: Site-Specific Protein Ubiquitylation Using an Engineered, Chimeric E1 Activating Enzyme and E2 SUMO Conjugating Enzyme Ubc9
    Article Snippet: .. To pSUMO1 (gift from Primo Schaer, Addgene #52258),53 genes of codon-optimized Ub with an N-terminal TEV-cleavable His6 tag, codon-optimized Ubc9 K14R and ChE1 v4.5 were cloned after each of the three operons. ..

    Plasmid Preparation:

    Article Title: SUMOylation of periplakin is critical for efficient reorganization of keratin filament network
    Article Snippet: .. Different PPL-C constructs were cloned in pGEX6P1 vector and cotransformed with pSUMO1 or pSUMO2 (Addgene). ..

    Article Title: Implications of critical nodes-dependent unidirectional cross-talk between Plasmodium and Human SUMO pathway proteins in Plasmodium infection
    Article Snippet: .. The Pf -SUMO wild type clone (pSUMO1-1S) for in bacto SUMOylation was derivatized from pSUMO1, was a gift from Primo Schaer (Department of Biomedicine, University of Basel; Addgene plasmid #52258). .. All Pf -SUMO and Pf -Ubc9 mutants were generated using Q5 Site-Directed Mutagenesis kit (NEB #E0554S) in Pf -SUMO wild type backbone.

    Article Title: Coronin1C SUMOylation modulates filopodia formation, neuritogenesis, and neuronal differentiation
    Article Snippet: .. The COR1C lysine mutants were generated using the Q5 Site-Directed Mutagenesis Kit (NEB) according to the manufacturer’s protocol. pSUMO1/2 was a gift from Primo Schaer (Department of Biomedicine, University of Basel; Addgene plasmid # 52258, #52259) . .. The following antibodies were used for Western blotting: anti-GST (1:5000, Hi-media), anti-His (1:2000, #BB-AB0010S, Biobharti), anti-SUMO1 (76-86, DSHB) anti-GFP (1:5000; #sc-9996 Santa Cruz Biotechnology), anti-GAPDH (1:3000; #10011 Abgenex), anti-COR1C (Proteintech-14749-1-AP), Goat anti-mouse AlexaFluor Plus 800 (1:40,000, #A32730, Thermo Fisher Scientific), Goat anti-rabbit AlexaFluor Plus 680 (1:40,000, #A32734 Thermo Fisher Scientific).

    Article Title: Klebsiella pneumoniae reduces SUMOylation to limit host defence responses
    Article Snippet: .. The following plasmids were used: pcDNA3 (Invitrogen), pSUMO1 (pcDNA3-HA-SUMO1 was a gift from Junying Yuan, Addgene plasmid # 21154), pSENP2-GFP (pEGFP-C2 SENP2 was a gift from Mary Dasso, Addgene plasmid # 13382), pSENP2-FLAG (FLAG-SENP2 was a gift from Edward Yeh, Addgene plasmid # 18047), GSK3β-WT (HA GSK3 beta wt pcDNA3 was a gift from Jim Woodgett, Addgene plasmid # 14753), GSK3β-S9A (HA GSK3 beta S9A pcDNA3 was a gift from Jim Woodgett, Addgene plasmid # 14754). .. Plasmids were purified from a host E. coli C600 strain using an Endofree Maxi-Prep kit from Qiagen (QIAGEN, catalog number 12362) following the manufacturer’s recommendations.

    Article Title: Klebsiella pneumoniae Reduces SUMOylation To Limit Host Defense Responses
    Article Snippet: .. The following plasmids were used: pcDNA3 (Invitrogen), pSUMO1 (pcDNA3-HA-SUMO1 was a gift from Junying Yuan, Addgene plasmid 21154), pSENP2-GFP (pEGFP-C2 SENP2 was a gift from Mary Dasso, Addgene plasmid 13382), GSK3β-WT (HA GSK3 beta wt pcDNA3 was a gift from Jim Woodgett, Addgene plasmid 14753), GSK3β-S9A (HA GSK3 beta S9A pcDNA3 was a gift from Jim Woodgett, Addgene plasmid 14754). .. Plasmids were purified from a host E. coli C600 strain using an Endofree Maxi-Prep kit from Qiagen according to the manufacturer’s recommendations.

    Article Title: Implications of critical node-dependent unidirectional cross-talk of Plasmodium SUMO pathway proteins.
    Article Snippet: .. The Pf-SUMO wild-type clone (pSUMO1-1S) for in bacto SUMOylation was derivatized from pSUMO1, and was a gift from Primo Schaer (Department of Biomedicine, University of Basel; Addgene plasmid #52258). .. All Pf-SUMO and Pf-Ubc9 mutants were generated using Q5 Site-Directed Mutagenesis kit (NEB #E0554S) in Pf-SUMO wild-type backbone.

    Article Title: Klebsiella pneumoniae Reduces SUMOylation To Limit Host Defense Responses
    Article Snippet: .. The following plasmids were used: pcDNA3 (Invitrogen), pSUMO1 (pcDNA3-HA-SUMO1 was a gift from Junying Yuan, Addgene plasmid 21154), pSENP2-GFP (pEGFP-C2 SENP2 was a gift from Mary Dasso, Addgene plasmid 13382), GSK3 -WT (HA GSK3 beta wt pcDNA3 was a gift from Jim Woodgett, Addgene plasmid 14753), GSK3 -S9A (HA GSK3 beta S9A pcDNA3 was a gift from Jim Woodgett, Addgene plasmid 14754). .. Plasmids were purified from a host E. coli C600 strain using an Endofree Maxi-Prep kit from Qiagen according to the manufacturer’s recommendations.

    Generated:

    Article Title: Coronin1C SUMOylation modulates filopodia formation, neuritogenesis, and neuronal differentiation
    Article Snippet: .. The COR1C lysine mutants were generated using the Q5 Site-Directed Mutagenesis Kit (NEB) according to the manufacturer’s protocol. pSUMO1/2 was a gift from Primo Schaer (Department of Biomedicine, University of Basel; Addgene plasmid # 52258, #52259) . .. The following antibodies were used for Western blotting: anti-GST (1:5000, Hi-media), anti-His (1:2000, #BB-AB0010S, Biobharti), anti-SUMO1 (76-86, DSHB) anti-GFP (1:5000; #sc-9996 Santa Cruz Biotechnology), anti-GAPDH (1:3000; #10011 Abgenex), anti-COR1C (Proteintech-14749-1-AP), Goat anti-mouse AlexaFluor Plus 800 (1:40,000, #A32730, Thermo Fisher Scientific), Goat anti-rabbit AlexaFluor Plus 680 (1:40,000, #A32734 Thermo Fisher Scientific).

    Mutagenesis:

    Article Title: Coronin1C SUMOylation modulates filopodia formation, neuritogenesis, and neuronal differentiation
    Article Snippet: .. The COR1C lysine mutants were generated using the Q5 Site-Directed Mutagenesis Kit (NEB) according to the manufacturer’s protocol. pSUMO1/2 was a gift from Primo Schaer (Department of Biomedicine, University of Basel; Addgene plasmid # 52258, #52259) . .. The following antibodies were used for Western blotting: anti-GST (1:5000, Hi-media), anti-His (1:2000, #BB-AB0010S, Biobharti), anti-SUMO1 (76-86, DSHB) anti-GFP (1:5000; #sc-9996 Santa Cruz Biotechnology), anti-GAPDH (1:3000; #10011 Abgenex), anti-COR1C (Proteintech-14749-1-AP), Goat anti-mouse AlexaFluor Plus 800 (1:40,000, #A32730, Thermo Fisher Scientific), Goat anti-rabbit AlexaFluor Plus 680 (1:40,000, #A32734 Thermo Fisher Scientific).



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